Clinical Article
Angiotensinogen Gene (M235T) Polymorphism and Coronary Artery Disease in the Egyptian Population. A genetic Association Study
Olfat Gamil Shaker, Heba Hamdy Eldemellawy, Hussien Heshmat Kassem.
Background: The etiology of coronary artery disease (CAD) involves both environmental and genetic factors; the former group was intensively studied. Angiotensinogen (AGT) is important in cardiovascular remodeling and control of blood pressure.
Methods: we investigated role of M235T variant of AGT gene in development of CAD. M235T refers to substitution of cytosine into thymine at codon 235 that encode for theronine instead of methionine at exon 2 of AGT gene.
Results: Seventy male patients were diagnosed as myocardial infarction (35) and unstable angina (35) were analyzed against healthy control male subjects (60) to evaluate gene polymorphism M235T and plasma angiotensinogen level. The distribution of genotype or paired allele among patients and control for 235TT variant was 23% and 3.3% and Odds Ratio (OR) was 8.83, for MT variant was 50% and 33.3% OR=2 and for MM was 27% and 63.3%, OR=0.21 respectively, there was significant difference between them as regard TT, MM genotype with P <0.01 and P <0.001 respectively. There was a significant increase in frequency of T allele distribution among cases than control P <0.001. There was a significant increase of plasma angiotensinogen level in patient than control P <0.01. Positive correlation was observed between TT genotype, age and diastolic blood pressure in CAD group r=0.34, P <0.001 and r=0.25, P <0.05 respectively. A significant increase of plasma angiotensinogen and cholesterol levels in CAD group than control was also observed.
Conclusions: T polymorphism of AGT gene was significantly associated with CAD and was correlated positively with blood pressure.
Keywords: Coronary artery
diseases, Angiotensinogen gene, Gene Variant.
(Heart Mirror J 2009; 3(2): 86-91).